Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.
Identifieur interne : 000062 ( Main/Exploration ); précédent : 000061; suivant : 000063Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.
Auteurs : Mohammed Najib Saïdi [Tunisie] ; Radhia Gargouri-Bouzid ; Mariem Rayanni ; Noureddine DriraSource :
- Molecular biotechnology [ 1073-6085 ] ; 2009.
Descripteurs français
- KwdFr :
- 2-Sulfanyl-éthanol (composition chimique), ARN des plantes (isolement et purification), ARN des plantes (métabolisme), Arecaceae (génétique), Banque de gènes (MeSH), Feuilles de plante (génétique), Flavonoïdes (métabolisme), Hybridation d'acides nucléiques (MeSH), Maladies des plantes (génétique), Octoxinol (MeSH), Phénols (métabolisme), Polyphénols (MeSH), Polyéthylène glycols (composition chimique), Povidone (composition chimique), Régulation de l'expression des gènes végétaux (MeSH).
- MESH :
- composition chimique : 2-Sulfanyl-éthanol, Polyéthylène glycols, Povidone.
- génétique : Arecaceae, Feuilles de plante, Maladies des plantes.
- isolement et purification : ARN des plantes.
- métabolisme : ARN des plantes, Flavonoïdes, Phénols.
- Banque de gènes, Hybridation d'acides nucléiques, Octoxinol, Polyphénols, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Arecaceae (genetics), Flavonoids (metabolism), Gene Expression Regulation, Plant (MeSH), Gene Library (MeSH), Mercaptoethanol (chemistry), Nucleic Acid Hybridization (MeSH), Octoxynol (MeSH), Phenols (metabolism), Plant Diseases (genetics), Plant Leaves (genetics), Polyethylene Glycols (chemistry), Polyphenols (MeSH), Povidone (chemistry), RNA, Plant (isolation & purification), RNA, Plant (metabolism).
- MESH :
- chemical , chemistry : Mercaptoethanol, Polyethylene Glycols, Povidone.
- chemical , isolation & purification : RNA, Plant.
- chemical , metabolism : Flavonoids, Phenols, RNA, Plant.
- genetics : Arecaceae, Plant Diseases, Plant Leaves.
- Gene Expression Regulation, Plant, Gene Library, Nucleic Acid Hybridization, Octoxynol, Polyphenols.
Abstract
A simple and efficient method was described here for the isolation of high-quality RNA from date palm leaves affected with Brittle Leaf Disease (BLD) and containing high amount of phenolic compounds. The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes.
DOI: 10.1007/s12033-008-9104-1
PubMed: 18815905
Affiliations:
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Le document en format XML
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(MeSH)</term><term>Mercaptoethanol (chemistry)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Octoxynol (MeSH)</term><term>Phenols (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Leaves (genetics)</term><term>Polyethylene Glycols (chemistry)</term><term>Polyphenols (MeSH)</term><term>Povidone (chemistry)</term><term>RNA, Plant (isolation & purification)</term><term>RNA, Plant (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>2-Sulfanyl-éthanol (composition chimique)</term><term>ARN des plantes (isolement et purification)</term><term>ARN des plantes (métabolisme)</term><term>Arecaceae (génétique)</term><term>Banque de gènes (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Flavonoïdes (métabolisme)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Octoxinol (MeSH)</term><term>Phénols (métabolisme)</term><term>Polyphénols (MeSH)</term><term>Polyéthylène glycols (composition chimique)</term><term>Povidone (composition chimique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Mercaptoethanol</term><term>Polyethylene Glycols</term><term>Povidone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Flavonoids</term><term>Phenols</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>2-Sulfanyl-éthanol</term><term>Polyéthylène glycols</term><term>Povidone</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arecaceae</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="génétique" 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with Brittle Leaf Disease (BLD) and containing high amount of phenolic compounds. The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Drira, Noureddine" sort="Drira, Noureddine" uniqKey="Drira N" first="Noureddine" last="Drira">Noureddine Drira</name><name sortKey="Gargouri Bouzid, Radhia" sort="Gargouri Bouzid, Radhia" uniqKey="Gargouri Bouzid R" first="Radhia" last="Gargouri-Bouzid">Radhia Gargouri-Bouzid</name><name sortKey="Rayanni, Mariem" sort="Rayanni, Mariem" uniqKey="Rayanni M" first="Mariem" last="Rayanni">Mariem Rayanni</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Saidi, Mohammed Najib" sort="Saidi, Mohammed Najib" uniqKey="Saidi M" first="Mohammed Najib" last="Saïdi">Mohammed Najib Saïdi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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